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大鼠胰十二指肠移植动物模型的显微外科技术改

日期:2018年01月15日 编辑: 作者:无忧论文网 点击次数:161206
论文价格:50元/篇 论文编号:2416 论文字数:2331 所属栏目:临床医学论文
论文地区: 论文语种:中文 论文用途:职称论文 Thesis for Title

大鼠胰十二指肠移植动物模型的显微外科技术改
基本信息
作者: 顾永平 黎介寿
专业: 
班级: 
研究方向: 
学校: 
指导老师: 
写作日期: 
字数: 2.331
编号: 52
类别: 医学〉临床医学
级别: 学术论文
摘要
目的:改进大鼠胰十二指肠移植动物模型,控制失血性休克的发生,增加术后成功率。
方法:供体门静脉同受体左肾静脉利用“卡夫”管相吻合。可以避免术中受体肝素化而导致血管吻合口渗血。给受体输注供体新鲜血2.5-3 ml.
结果:65例药物诱导糖尿病大鼠行胰十二指肠移植。60例成活超过7天,成功率92.3%,非禁食情况下,血糖浓度均正常。
结论:该模型的技术改进减少许多并发症,大大提高了手术成功率。
主题词: 胰十二指肠移植, “卡夫”管吻合, 输血, 大鼠
Refined Technique for Pancreaticoduodenal Transplantation with Systemic Venous Drainage and Enteric Drainage in the Rat
Gu Yongping, Li Jieshou 
Transplant Center Lab of the Chinese People’s Liberation Army, Department of Surgery, Nanjing General Hospital, Medical School of Nanjing University, 305, East Road of Zhongsan, Nanjing 210002, China
Address reprint requests to Gu Yongping, Transplant Center Lab of PLA, Department of Surgery, Nanjing General Hospital, Medical School of Nanjing University, 305, East Road of Zhongsan, Nanjing 210002, China
The work was supported by grants form the Key National Science and Technology Research, project 96Z-002
ABSTRACT
Objective To refine the technique of Lee’s pancreaticoduodenal transplantation in the rat1, to overcome hypovolemic shock, to enhance the success rate.
Methods We reconstructed the venous anastomosis by a “cuff” anastomosis between the donor portal vein and the recipient renal vein. Meanwhile, we injected the recipient 2.5-3 ml fresh blood which was taken form the donor. 
Results Of 65 transplanted rats that were induced diabetes, 60 rats survived over 7 days, the success rate was 92.3%. All success rats were euglycemia. 
Conclusions Our fefined techniques could eliminate most surgical complications, allowing the pancreaticoduodenal transpiantations in the rat with a high degree of success. 
Keywords: refined technique; pancreaticoduodenal transplantation; a “cuff” anastomosis; blood infusion; rat

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